You might be a research scientist wishing to make a peptide and
antibody for probing a protein's structure and function, or one
developing a vaccine or an immunodiagnostic test,
or you might be an antibody producing
company wishing to expand its product portfolio.
At the outset of any of
these pursuits, possibly the most frequently expressed concern is
which peptide sequences to choose for generating antibodies.
you may be experiencing difficulty producing antibodies to a purified
protein and even be considering artificially tagging the target protein with an unrelated epitope.
Or, you may be frustrated by the lack of commercially
available antibodies to regions other than protein N- and C-termini,
and, thus, be considering producing your own antibodies.
You may even wish to produce a library of peptides
and antibodies to target specific regions of a protein of interest.
Also, you may be unsure of the thoroughness of an epitope prediction
service that is bundled with a peptide synthesis or epitope mapping
In any of these situations you might be faced with choosing between
self-performed epitope prediction and analysis, which is
difficult, or enlisting assistance from experts such as ePitope
ePitope Informatics specialises in in silico epitope discovery
and related protein annotation, with a particular focus being epitope prediction and target protein
epitope contextual analysis for the identification and targeting of
antibody epitopes. ePitope Informatics is also research-active in
Our solution is to provide in silico epitope discovery services that
address issues including:
accuracy, and the
that it is better to
arrive at a consensus
through use a variety of
algorithms and structure
The evidence for certain
algorithms being better
epitopes in order of likely
Weighting given to a
region predicted to be
predicted to be antigenic,
or vice versa.
settings of certain
algorithms, and the
possibility of adjusting
these (which changes the
the algorithm) to obtain
Degree of importance
attached to findings of
epitopes located on solved
The presence of amino
acid sequence motifs and
protease cleavage sites
providing further evidence
that a predicted epitope is
surface-located on a target
identified by searching
databases for sequences
showing identity to
The effect of
(in epitope query
sequences) on primary
protein database searching.
search parameters that are
recommended for use with
(e.g. predicted epitopes).
amino acid signature
protein databases and
predicted epitope query
sequences, which can
alignments to be missed.